tandem software Search Results


tandem repeat software  (Lawrence Livermore National Security LLC)
90
Lawrence Livermore National Security LLC tandem repeat software
Tandem Repeat Software, supplied by Lawrence Livermore National Security LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/10__1097_slash_olq__0000000000000935-21-35-38?v=Lawrence+Livermore+National+Security+LLC
Average 90 stars, based on 1 article reviews
tandem repeat software - by Bioz Stars, 2026-08
90/100 stars
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tandem mass spectrometry data  (GraphPad Software Inc)
90
GraphPad Software Inc tandem mass spectrometry data
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Tandem Mass Spectrometry Data, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/pmc08877175-131-0-21?v=GraphPad+Software+Inc
Average 90 stars, based on 1 article reviews
tandem mass spectrometry data - by Bioz Stars, 2026-08
90/100 stars
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mini electrodes tandem data acquisition software  (BIOPAC)
90
BIOPAC mini electrodes tandem data acquisition software
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Mini Electrodes Tandem Data Acquisition Software, supplied by BIOPAC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/pm36290615-79-6-12?v=BIOPAC
Average 90 stars, based on 1 article reviews
mini electrodes tandem data acquisition software - by Bioz Stars, 2026-08
90/100 stars
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two mini electrodes tandem data acquisition software  (BIOPAC)
90
BIOPAC two mini electrodes tandem data acquisition software
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Two Mini Electrodes Tandem Data Acquisition Software, supplied by BIOPAC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/pmc09598376-68-6-12?v=BIOPAC
Average 90 stars, based on 1 article reviews
two mini electrodes tandem data acquisition software - by Bioz Stars, 2026-08
90/100 stars
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tandem mass spectrometer with electrospray interface, quattro lcz and masslynx software  (Micromass UK Limited)
90
Micromass UK Limited tandem mass spectrometer with electrospray interface, quattro lcz and masslynx software
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Tandem Mass Spectrometer With Electrospray Interface, Quattro Lcz And Masslynx Software, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/pm16332629-63-28-30?v=Micromass+UK+Limited
Average 90 stars, based on 1 article reviews
tandem mass spectrometer with electrospray interface, quattro lcz and masslynx software - by Bioz Stars, 2026-08
90/100 stars
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openeye's tandem rocs  (OpenEye Scientific Software Inc)
90
OpenEye Scientific Software Inc openeye's tandem rocs
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Openeye's Tandem Rocs, supplied by OpenEye Scientific Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/pm20359237-209-23-7?v=OpenEye+Scientific+Software+Inc
Average 90 stars, based on 1 article reviews
openeye's tandem rocs - by Bioz Stars, 2026-08
90/100 stars
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tandem aspartate  (Proteome Software Inc)
86
Proteome Software Inc tandem aspartate
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Tandem Aspartate, supplied by Proteome Software Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/pm40910777-268-0-15?v=Proteome+Software+Inc
Average 86 stars, based on 1 article reviews
tandem aspartate - by Bioz Stars, 2026-08
86/100 stars
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x tandem v2017 2 1 4  (Proteome Software Inc)
86
Proteome Software Inc x tandem v2017 2 1 4
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
X Tandem V2017 2 1 4, supplied by Proteome Software Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/10__1074_slash_jbc__ra119__010486-416-13-8?v=Proteome+Software+Inc
Average 86 stars, based on 1 article reviews
x tandem v2017 2 1 4 - by Bioz Stars, 2026-08
86/100 stars
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tandem mass spectrometry analyses  (Xcalibur Software Inc)
86
Xcalibur Software Inc tandem mass spectrometry analyses
Heat-map-based mass <t>spectrometry</t> analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.
Tandem Mass Spectrometry Analyses, supplied by Xcalibur Software Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tandem+software/10__1523_slash_jneurosci__4067___10__2010-110-1-14?v=Xcalibur+Software+Inc
Average 86 stars, based on 1 article reviews
tandem mass spectrometry analyses - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


Heat-map-based mass spectrometry analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.

Journal: International Journal of Molecular Sciences

Article Title: Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears

doi: 10.3390/ijms23042300

Figure Lengend Snippet: Heat-map-based mass spectrometry analysis of sphingolipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingolipid isoforms: ceramides (Cer), dihydroceramides (DHCer), lactosylceramides (LacCer), and monohexosylceramides (HexCer) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurons at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction, and the red colour refers to an increase of lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Both hippocampal neurones and glial cells showed an increased and reduced intensity of Cer, DHCer, LacCer, and HexCer isoforms after both 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment. Different changes in the lipid classes are shown between treated hippocampal neurones and glial cells. Aβ-treated hippocampal neurones showed prominent increases and decreases in Cer isoforms and LacCer and HexCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. There was both a prominent intensity increase and a decrease of DHCer isoforms after 3 h and 12 h of Aβ (1–40) and Aβ (1–42) treatment. Glial cells showed prominent lipid changes of Cer, DHCer, LacCer, and HexCer isoforms after 3 h and 12 h of Aβ (1–40) or Aβ (1–42) treatment.

Article Snippet: Tandem mass spectrometry data of three independent experiments ( n = 3) from neurones and glial cells were further processed by GraphPad Prism 7, performing logarithmic 10 transformation, and plotted to our negative control (DMSO).

Techniques: Mass Spectrometry, Negative Control

Heat-map-based mass spectrometry analysis of sphingophospholipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingophospholipid isoforms; sphingosine (d18:1 So), sphinganine (d18:0 Sa), sphingosine-1-phosphate (d18:1 So1P), sphinganine-1-phosphate (d18:1 Sa1P), sphingomyelines (SM) and dihydrosphingomyelines (DHSM) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) hippocampal neurones at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction and the red colour refers to an increase in lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Major intensity changes in lipid classes of d18:1 So, d18:0 Sa, d18:1 So1P, d18:1 Sa1P after 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment of hippocampal neurones and glial cells were observed. Specific lipid intensity changes after Aβ (1–40) and Aβ (1–42) treatment of hippocampal neurones of very long fatty acid SM compared to Aβ-treated glial cells were detected.

Journal: International Journal of Molecular Sciences

Article Title: Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears

doi: 10.3390/ijms23042300

Figure Lengend Snippet: Heat-map-based mass spectrometry analysis of sphingophospholipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different sphingophospholipid isoforms; sphingosine (d18:1 So), sphinganine (d18:0 Sa), sphingosine-1-phosphate (d18:1 So1P), sphinganine-1-phosphate (d18:1 Sa1P), sphingomyelines (SM) and dihydrosphingomyelines (DHSM) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) hippocampal neurones at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes of these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction and the red colour refers to an increase in lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Major intensity changes in lipid classes of d18:1 So, d18:0 Sa, d18:1 So1P, d18:1 Sa1P after 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment of hippocampal neurones and glial cells were observed. Specific lipid intensity changes after Aβ (1–40) and Aβ (1–42) treatment of hippocampal neurones of very long fatty acid SM compared to Aβ-treated glial cells were detected.

Article Snippet: Tandem mass spectrometry data of three independent experiments ( n = 3) from neurones and glial cells were further processed by GraphPad Prism 7, performing logarithmic 10 transformation, and plotted to our negative control (DMSO).

Techniques: Mass Spectrometry, Negative Control

Heat map-based mass spectrometry analysis of glycerophospholipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different glycerophospholipids isoforms: phosphatidylcholine (PC), lyso-phosphatidylcholine (LPC), lyso-phosphatidylethanolamine (LPE), lyso-phosphatidylglycerol (LPG), lyso-phosphatidylserine (LPS), and lyso-platelet-activating factor (Lyso-PAF) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurones at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes in these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction and the red colour refers to an increase in lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Hippocampal neurones specifically showed PC (38:0) intensity changes after 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment compared to an overall reduction in PC for Aβ (1–40) and Aβ (1–42) -treated glial cells. LPC, LPG, LPS, and Lyso-PAF showed specific changes after 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment in both hippocampal neurones and glial cells.

Journal: International Journal of Molecular Sciences

Article Title: Aβ-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears

doi: 10.3390/ijms23042300

Figure Lengend Snippet: Heat map-based mass spectrometry analysis of glycerophospholipid classes from hippocampal neurones and glial cells after 3 h and 12 h Aβ treatment. Here, we examined different glycerophospholipids isoforms: phosphatidylcholine (PC), lyso-phosphatidylcholine (LPC), lyso-phosphatidylethanolamine (LPE), lyso-phosphatidylglycerol (LPG), lyso-phosphatidylserine (LPS), and lyso-platelet-activating factor (Lyso-PAF) after 3 h and 12 h treatment with (1 µM) Aβ (1–40) and (1 µM) Aβ (1–42) of hippocampal neurones at DIV 12 and glial cells (all groups, from three independent experiments ( n = 3)). Changes in these lipid classes are shown as logarithmic (log 10 ) relative intensity (arbitrary unit); the green colour refers to a reduction and the red colour refers to an increase in lipid levels compared to our negative control (DMSO), which was set to 0 as baseline. Hippocampal neurones specifically showed PC (38:0) intensity changes after 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment compared to an overall reduction in PC for Aβ (1–40) and Aβ (1–42) -treated glial cells. LPC, LPG, LPS, and Lyso-PAF showed specific changes after 3 h and 12 h Aβ (1–40) and Aβ (1–42) treatment in both hippocampal neurones and glial cells.

Article Snippet: Tandem mass spectrometry data of three independent experiments ( n = 3) from neurones and glial cells were further processed by GraphPad Prism 7, performing logarithmic 10 transformation, and plotted to our negative control (DMSO).

Techniques: Mass Spectrometry, Negative Control